CRISPR and Zinc Finger Injections

Zinc FingerCRISPR technologies allow for the modification of the endogenous gene locus by insertions, deletions, or mutations. CRISPR, and ZFNs have become important scientific tools for mutagenesis and genomic modification.

For customers looking to create these targeted mutations, Genetic Services, Inc. will perform zinc finger nucleus, and CRISPR injections for both Drosophila and mosquito species.

Injection Only Service

Currently, we offer a Drosophila and mosquito embryo injection service for zinc finger nuclease and CRISPR injections.

Customers can choose from homologous recombination (HR) or non-homologous end joining (NHEJ) for injection only services. We inject the mRNA with either a donor plasmid or oligo and ship the injected flies.

For every construct, we request that our customers send 30-50 micrograms of Qiagen-purified DNA (if applicable) and several aliquots of mRNA (with each aliquot containing 5-10 microliters).

Upon receipt from the customer of DNA and mRNA for each construct, Genetic Services, Inc. will perform the following:

  1. Purify the DNA prior to injections using our in house technique.
  2. Combine the DNA and mRNA immediately prior to injection.
  3. Collect and ship the injected embryos/larvae.

Please Contact Us for pricing and current promotions.

Mutation Screening Service

We are now offering mutation screening service for CRISPR and ZFN injections. 

For homologous recombination projects, we offer mutation screening for visible and non-visible markers.  

For non-homologous end joining projects, we offer mutation screening at three stages:

  1. Injection and mutation screening of NHEJ with a mixture of mutations (We will ship at least 3 vials of flies containing one or more mutation per vial).
  2. Injection and mutation screening of NHEJ with separated mutations (We will ship at least 3 vials of flies containing a single mutation).
  3. Injection and mutation screening of NHEJ with separated mutations and sequencing (We will ship at least 3 vials of flies containing a single mutation with sequencing).

For every construct, we request that our customers send 30-50 micrograms of Qiagen-purified DNA (if applicable) and several aliquots of mRNA (with each aliquot containing 5-10 microliters).

Upon receipt from the customer of DNA and mRNA for each construct, Genetic Services, Inc. will perform the following:

  1. Purify the DNA prior to injections using our in house technique.
  2. Combine the DNA and mRNA immediately prior to injection.
  3. Cross and screen flies to desired level.
  4. Ship mutated flies.

Please Contact Us for pricing and current promotions or look at our pricing information.

Terms of Purchase
  1. Payment terms of 30 days net invoice
  2. Price excludes shipping and handling
  3. Customer should forward the DNA sample to the following:

    Genetic Services, Inc.

    16 Craig Rd.
    Sudbury, MA 01776
    Attn: S. Zusman

    We accept VISA & MASTERCARD and Purchase Order numbers.