Fosmid & BAC Injections

FosmidGenetic Services, Inc. has developed innovative methods of DNA preparation and Drosophila embryo injection to greatly increase the transformation efficiency for Fosmid and BAC constructs between 30 and 120 kb. In the past year, this technology has allowed us successfully transform hundreds of large constructs quickly and efficiently.

Additionally, we offer our customers DNA preparation services for Fosmid and BAC constructs. Just send us your bacterial plate or stab and our highly skilled scientists and technicians will prep the DNA for you quickly with our in-house technique. Then, we can quickly inject the constructs before any degradation can occur.

Please note that large constructs (over 35kb) transform at reduced rates (only around 1-3% on average) compared to constructs smaller than 35kb, which transform at a rate of 10-20%. Thus, for these constructs, we inject two to three times as many embryos in order to guarantee success.

Injection Only Service
For customers that would like to do their own screening, we offer a Drosophila embryo injection service for Fosmid and BAC injections.

Upon receipt from the customer of either bacterial plates/stabs or 30-50 micrograms of Qiagen-purified DNA for each construct, Genetic Services, Inc. will perform the following:

  1. Prep the DNA from the plates/stabs or purify the already prepped DNA prior to injections using our in house technique
  2. Collect and ship enough larva for at least 1-3 individual transgenic lines per construct

Transgenic Production Service
We additionally offer transgenic production services for Fosmid and BAC injections.

Upon receipt from the customer of either bacterial plates/stabs or 30-50 micrograms of Qiagen-purified DNA for each construct, Genetic Services, Inc. will perform the following:

  1. Prep the DNA from the plates/stabs or purify the already prepped DNA prior to injections using our in house technique
  2. Collect the injected embryos and rear them to adulthood
  3. Cross the G0 injected adults to w1118 (or appropriately marked) flies and then collect transformant flies to generate independent lines. We guarantee at least 1-3 individual transformant lines
  4. Maintain copies of the transformant lines until they are growing well in the customer's lab

Uniquely Designed Landing Sites for Fosmid and BACs
Ask us about our uniquely designed Drosophila landing-site strains for large constructs. These strains have been shown to greatly increase the transformation efficiency, so we highly recommend that our customers consider them for their Fosmid and BAC injections.

For more information about these landing-sites, please Contact Us

Additional Services

Mapping and Balancing Service
In addition to obtaining transgenics for your constructs, we also offer mapping and homozygousing services for Fosmid and BAC constructs. We will balance as many lines as you would like and ship you the balanced lines a few weeks after we have shipped the transgenic lines.

Virgin Collection Service
Tired of collecting virgin flies to cross to the G0 flies arising from your injected larvae or transformant vials? We will collect and ship you 100 virgin flies ten days after we inject your larvae or ship you transgenic lines. We can send virgin flies for the following stocks:

  • w[1118]
  • y,w
  • y,v
  • y,sc,v
  • amos/cyo
  • Dr e/TM3

Terms of Purchase:

  1. Payment terms of 30 days net of invoice
  2. Price excludes Shipping and Handling
  3. Customer should forward the DNA sample to the following:

Genetic Services, Inc.

16 Craig Rd.
Sudbury, MA 01776
Attn: S. Zusman

We accept VISA & MASTERCARD and Purchase Order numbers.

Contact Us for pricing and current promotions.

First time users can take advantage of our special discount of 10% off your first order.