Our Pricing

At Genetic Services Inc., we are not only dedicated to offering the highest quality of service, but also the most competitive price in the industry.

We have recently lowered our injection prices and now offer some of the lowest injection rates around. Furthermore, we have a total satisfaction guaranteed policy for all of our customers!

We are additionally offering a variety of new services. We strive to be both constantly improving the quality of our services and expanding them to continually meet the Drosophila communities’ needs.

We are the only injection company where all of our Drosophila melanogaster P-element and site directed injections are guaranteed!

We do everything at our Cambridge, Massachusetts location and make customer satisfaction our number #1 priority.

P-Element Injection Services

Site-Directed Injection Services

Zinc Finger Injections

Large Construct Injections

CRISPR Production and Injections

MIMIC and RCME Injections

Non-melanogastor Injection Services

Additional Services

 

 P-element Injection Services

 

Injection Only Service: $225

This service includes preparation and injection of DNAs into Drosophila melanogaster and shipping of injected larvae . At least 5 transformed Drosophila lines per construct are guaranteed.

Transgenic Service: $299

For our transgenic service, we will inject your construct, cross out the injected flies, and then screen and ship you the transgenics. We guarantee a minimum of 5 independent transgenic lines!

Balancing and Homozygousing Service: Please Contact Us for more details

On top of screening for transgenics, we will additionally balance and homozygous your lines.

 

 Site-Directed Injection Services

 

Injection Only Service for constructs < 30 kb: $199

This service includes preparation and injection of DNAs into Drosophila melanogaster and shipping of injected larvae. At least 1-3 transformed Drosophila lines per construct are guaranteed.

Transgenic Service for constructs < 30 kb: $299

This service includes injection of a DNA constructs into Drosophila embryos with an attp target site using site directed phiC31 integrase technology.   At least 1-3  transformed Drosophila lines per construct are guaranteed.

Balancing and Homozygousing Service for constructs < 30 kb: $50/construct

On top of screening for transgenics, we will additionally balance and homozygous your lines.

 

 Large Construct Injections (BACs and Fosmids > 30kb)

 

Large Construct Injection Only Service: Please contact us for pricing information.

We will inject your BAC or fosmid into the landing site of your preference and ship you the injected larvae shortly thereafter.

We recommend a 3X injection (require a minimum of 2X) for large constructs over 30kb. We have developed a unique method of DNA preparation and injection that allows us to get extremely high transformation rates for large constructs.

Please note that we strongly recommend that you allow us to prep your DNA from your plate/stab as we have had very good transformation with our in-house DNA preparation technique. Also, ask us about our specially designed landing sites for large constructs!

Transformants are guaranteed for a 3X injection!

Large Construct Transgenic Service: Please contact us for pricing information.

We will inject your BAC or fosmid into the landing site of your preference, cross out the injected flies, and then screen and ship you the transgenics.

We recommend a 3X injection (require of minimum of 2X) for large constructs over 30kb. We have developed a unique method of DNA preparation and injection that allows us to get extremely high transformation rates for large constructs.

Please note that we strongly recommend that you allow us to prep your DNA from your plate/stab as we have had very good transformation with our in-house DNA preparation technique. Also, ask us about our specially designed landing sites for large constructs!

Transformants are guaranteed for a 3X injection!

 

 Zinc Finger Injections

 

Zinc finger Injection Only Service: $399

This service includes injection of the mRNA (with or without donor plasmid) into appropriate Drosophila strain, and shipment of larvae shortly thereafter.

Transgenic zinc finger Injection Only  Service (with donor plasmid): $599

This service includes injection of donor plasmid and mRNA into appropriate Drosophila strain, and screening for a site specific break and insertion by homology-directed repair (must have visible marker).

 

CRISPR Production 

 

CRISPR gsRNA template design and production- $399.00

For this service please send at least a 100 bp sequence of the region you would like the CRISPR made against.

For a multiple CRISPR gsRNA template design project, significant discounts are available, please contact us for more details.

 

mRNA production $199.00

mRNA by in vitro transcription for either our generated or a lab provided CRISPR gsRNA template.

*Please note that billing for CRISPR production and design occurs when the order is received and billing for any CRISPR screening occurs 60 days later, resulting in two bills.

 

CRISPR Homologous Recombination (HR) Injections into Cas9 fly line

 

Injection only service- $399.00

We inject the CRISPR with either a donor plasmid or oligo and ship the injected flies.

Injection and mutation screening of HR injection with visible marker $499.00

Injection and mutation screening of HR, NO visible marker - please contact us for pricing

We will ship at least 1 vial of flies containing one or more mutations per vial.

*The customer will be billed 60 days after the order is received.

 

CRISPR Non Homologous End Joining (NHEJ) Injections into Cas9 fly line

 

Injection only service- $399.00

We inject the CRISPR and ship the injected flies.

Injection and mutation screening of NHEJ with a mixture of mutations $699.00

We will ship at least 1 vial of flies containing one or more mutations per vial.

Injection and mutation screening of NHEJ with separated balanced mutations $999.00

We will ship at least 1 vial of flies containing a single mutation.

Sequencing of NHEJ separated mutations $100.00/line

If your mutation is not lethal when homozygoused, we will homozygous your flies and sequence. 

*The customer will be billed 60 days after the order is received.

 

MiMIC Injections 

 

MiMIC Injection Only Service: $399

This service includes preparation and injection of DNA constructs into the appropriate MiMIC Drosophila melanogaster strain crossed to a balancer strain containing an integrase source and shipment of larvae shortly thereafter.

MiMIC Transgenic Service: $599

This service includes preparation and injection of DNA constructs into the approriate MiMIC Drosophila melanogaster strain crossed to a balancer strain containing an integrase source, crossing the flies that emerge from the injection, and screening for the appropriate marker.

 

Recombinase Mediated Cassette Exchange (RMCE)


RMCE Injection Only Service: $399

This service includes preparation and injection of DNA constructs into the appropriate Drosophila mealnogaster embryos and shipping the injected larvae.

RMCE Transgenic Service: $499

This service will include preparation and injection of DNA constructs into the appropriate Drosophila melanogaster embryos, crossing the flies that emerge from the injection, and screening for the appropriate marker.

 

Non-melanogastor Injection Services 

 

Injection Only Service: $399

This service includes preparation and injection of DNA constructs into embryos of a non-melanogaster Drosophila species and shipping the injected larvae.

Transgenic Service: $599

This service includes preparation and injection of DNA constructs into embryos of a non-melanogaster Drosophila species, crossing the flies that emerge from the injection, screening for the appropriate marker, and shipping transgenics.

 

DNA Preparation Service 

Just send us a stab/plate and we will midiprep your DNA prior to injection using our in-house technique. We strongly recommend that we prep your DNA if your construct > 30kb.

  • Small construct (under 30kb): $35/prep
  • Constructs 30-80kb: $69/prep
  • Constructs > 80kb: $89/prep

 

PCR Verification Of Your Insertion

After obtaining transgenics for your construct, we will PCR verify that your insertion is in the correct location. The price for this is $50 + $10 per line verified.

 

Fluorescent Screening

We will screen various fluorescent markers including GFP, RFP, and CFP for $50/construct.

 

Vermillion Screening

We will screen for vermillion phenotype for $50/construct.

 

Virgin Collection Service

We will collect and ship you 100 virgin flies 10 days after we either collect your larvae or ship your transgenic lines. The price of this service is $75. Please contact us if you would like additional virgins shipped.

 

New Strain

We have a $100/melanogaster strain and $150/non-melanogaster strain handling/setup fee to grow up your strain and maintain it for 2-3 months. We will not charge you another setup fee for additional orders during the six month timeframe that we have your strains.

*Please note that all new stocks are placed in our quarantine for several weeks before we begin the injections.

 

Shipping and Handling

Shipping

We ship all orders either overnight priority or international priority to ensure that your larvae or flies arrive in good condition. Our shipping charges are:

  • $49 for Domestic shipping.
  • $69 for Canada
  • $79 for International (Mexico, South America, Europe, Asia, Australia, and Africa)

Handling

We charge a $35 handling fee per order regardless of how many constructs are in your order.

 

Molecular Biological Services

Primer Design and Construct Production

Please contact us for pricing information.